目的 研究中药九里香醇提物中抗肿瘤活性的贡献成分。方法 采用MTT和SRB法测定九里香醇提物 (1~100 μmol·L-1)对U937,HL-60,K562,Bel7402,Hela 5种人肿瘤细胞的生长抑制活性。运用常规色谱方法对活性成分进行追踪分离,采用现代谱学技术鉴定化合物的结构,并对分离得到的化合物进行体外活性测定。结果 在给药48 h后,部分醇提物随着给药浓度的增加,肿瘤细胞的存活率明显下降, 在醇提物的A8和A9部分共归属并鉴定出九里香抗肿瘤有效部位的活性化合物4 个,分别为异落叶松脂醇 (Ⅰ),二甲氧基异落叶松脂醇 (Ⅱ),3-甲氧基异落叶松脂醇 (Ⅲ)和5′-甲氧基异落叶松脂醇 (Ⅳ)。结论 化合物Ⅰ和Ⅱ可能为九里香醇提物中抗肿瘤作用的活性成分,化合物Ⅰ~Ⅳ为首次从该植物中分离得到。
Abstract
OBJECTIVE To investigate the cytotoxic activities of chemical constituents in alcohol extract of the stem bark of Murraya exotica L. METHODS The cytotoxicity against five cancer cell lines, U937,HL-60,K562,Bel7402 and Hela, were assayed by MTT and SRB METHODS. The constituents were isolated from Murraya exotica L. by routine chromatographic METHODS and the structures of the isolates were elucidated by NMR techniques. The antitumor effect was evaluated on cancer cells in vitro. RESULTS When the cancer cells were exposed to the extract for more than 48 h, the survival rate decreased with the increase of the drug concentration. Four compounds were isolated and identified as isolariciresinol (Ⅰ), dimethoxy isolariciresinol (Ⅱ), 3-methoxyisolariciresinol (Ⅲ), and 5′-methoxyisolariciresinol (Ⅳ). CONCLUSION The active ingredients in Murraya exotica L. have antitumor activities, which may be compounds Ⅰand Ⅱ.
关键词
九里香 /
醇提物 /
抗肿瘤 /
异落叶松脂醇 /
二甲氧基异落叶松脂醇
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Key words
Murraya exotica L. /
alcohol extract /
antitumor /
isolariciresinol /
dimethoxy isolariciresinol
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中图分类号:
R284
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参考文献
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脚注
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